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Image Search Results
Journal: Frontiers in Microbiology
Article Title: Detecting Lactococcus lactis Prophages by Mitomycin C-Mediated Induction Coupled to Flow Cytometry Analysis
doi: 10.3389/fmicb.2017.01343
Figure Lengend Snippet: Representative cytograms for SYTO9-stained samples of two L. lactis controls using the BD Accuri TM C6 flow cytometer. (A,C) Cytograms of MmC-treated L. lactis UC509.9 (prophage-free lactococcal strain used as negative control); (B,D) Cytogram of MmC-treated L. lactis NZ9000-TP901-1 erm (lactococcal strain harboring the TP901-1 prophage used as a positive control); (A,B) Representative cytograms of the gating strategy for size discrimination between the phage-containing (induced) sample (Gate 1, red) and the control 6.0 μm microsphere suspension (Gate 2, blue), using SSC (Side Scattered light) versus FSC (Forward Scattered light) analysis. (C,D) Representative cytogram for the measurement of fluorescence detection (FL1/SYTO-9) in the phage population versus SSC, identified in Gate 1 in (A,B) mentioned above, which was subsequently selected in Gate 3 (purple). Gate 3 was applied to identify the percentage of fluorescence detected by phage DNA- containing particles stained with SYTO-9. (E) Percentage of SYTO-9 fluorescence detected in the Gate 3 applied in two inducible L. lactis strains (positive and negative control) using 3 μg.ml -1 of MmC.
Article Snippet: FCM analysis was performed using the
Techniques: Staining, Flow Cytometry, Negative Control, Positive Control, Fluorescence